Filtering Homebrewed Gear

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It seems to be the hombrew standard to use 0.45 micron filters when converting powders. The most recent examination of this practice suggests that a 0.2 micron would be better. The reason being that some types of bacteria (like staph) go into a dormant spore state when growth conditions are not optimal. These spores average about 0.3 microns in diameter and will pass through a 0.45 micron filter. Spores are some of the most hardy things on the planet. They can withstand extreme heat, cold and even harsh chemical bactereostatic agents like straight bleach. Trust me when I say the piddly little 3% to 10% BA in a mixture won't do Jack and they can handle heat that would decompose your gear. When the spores are deposited in an environment with adequate water, nutriuents, and temperature (like say your muscle bed), they come back to life and voila, you have a staph infection. A staph infection in your muscle bed can be extremely serious; it could lead to amputation or even death. Why are so many people willing to risk this scenareo by relying on baking, BA, and 0.45 micron filters?  

23 May 2004 12:49
I have a question.....

I normally use either a filtration unit like what is offered by Labrotory Lab Supply Inc. or I use the coffee filter and jar method which has always made crystal clear oils for me in the past; never any problems.

When I first got started, I tried a .45 whatman filter attached to a large syringe. It was nearly impossible to push 1cc thru this filter. Am I doing something wrong? maybe the density/thickness of the oil was too much for the filter???

Or maybe the .45 whatman filters need to be used when making test susp or winny.
Please let me know guys...if there is a trick to this or something Im doing wrong please lemme know. :thumbs:  

10 Sep 2007 00:29
Good post Candle  

31 Jul 2009 01:07

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